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Protective effect of green tea polyphenol (‐)‐epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates
Author(s) -
Lee SeongRyong,
Im KiJo,
Suh SeongIl,
Jung JungGil
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1090
Subject(s) - lipid peroxidation , trolox , chemistry , antioxidant , biochemistry , ferrous , pro oxidant , oxidative stress , epicatechin gallate , epigallocatechin gallate , polyphenol , organic chemistry , dpph
The aim of this study was to compare the protective effects of green tea polyphenol (‐)‐epigallocatechin gallate (EGCG) and other well‐known antioxidants on the lipid peroxidation in gerbil brain homogenates. Oxidative stress was induced by H 2 O 2 (10 mM) or ferrous ammonium sulfate (5 µM) and lipid peroxidation was studied. Hydrogen peroxide and ferrous ions are capable of oxidizing a wide range of substrates and causing biological damage. The reaction, referred to as the Fenton process, is complex and can generate both hydroxyl radicals and higher oxidation states of the iron. Thiobarbituric acid‐reactive substances (TBA‐RS) were used as a marker of lipid peroxidation. EGCG, trolox, lipoic acid, and melatonin reduced H 2 O 2 ‐ or ferrous ion‐induced lipid peroxidation in a concentration‐dependant manner. In reducing the H 2 O 2 ‐induced lipid peroxidation, IC 50 values of antioxidants were as follows: EGCG (0.66 µM), trolox (37.08 µM), lipoic acid (7.88 mM), and melatonin (19.11 mM). In reducing the ferrous ion‐induced lipid peroxidation, IC50 values of antioxidants were as follows: EGCG (3.32 µM), trolox (75.65 µM), lipoic acid (7.63 mM), and melatonin (15.48 mM). Under the in vitro conditions of this experiment, EGCG was the most potent antioxidant in inhibiting H 2 O 2 or ferrous ion‐induced lipid peroxidation in the gerbil brain homogenates. Copyright © 2003 John Wiley & Sons, Ltd.