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Antioxidant effect of polyamines on erythrocyte cell membrane lipoperoxidation after free‐radical damage
Author(s) -
Farriol Mireia,
SegoviaSilvestre Toni,
Venereo Yusimy,
Orta Xavi
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1073
Subject(s) - spermidine , spermine , putrescine , polyamine , tbars , malondialdehyde , antioxidant , chemistry , thiobarbituric acid , lipid peroxidation , biochemistry , hydrogen peroxide , pharmacology , endocrinology , biology , enzyme
This in vitro study determined the effect of three doses each (100, 500 and 1000 μm) of putrescine, spermidine and spermine on malondialdehyde (MDA) release in red blood cells (RBCs) from healthy individuals after hydrogen peroxide stimulation (10 m M ). Twenty‐two volunteers, 9 males and 13 females, aged 41.5 ± 16.4 years, were studied. MDA was measured by thiobarbituric reaction (TBARs) and the results were calculated using ϵ = 1.56 × 10 5 . The analysis of variance (ANOVA) demonstrated a statistically significant overall decrease in MDA release in the polyamine‐exposed cells ( p < 0.0001) when compared with unexposed cells. Individual analysis of each polyamine separately showed a 52% decrease in MDA release with added spermine and a 39.5% decrease with added spermidine ( p < 0.001). No evaluable effect was found for putrescine. There was no correlation between the effect produced and the three doses of spermidine or spermine added, indicating a non dose‐dependent action. Copyright © 2003 John Wiley & Sons, Ltd.