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Effect of ganhuangenin obtained from Scutellaria radix on the chemical mediator production of peritoneal exudate cells and immunoglobulin E level of mesenteric lymph node lymphocytes in Sprague‐Dawley rats
Author(s) -
Lim Beong Ou
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1034
Subject(s) - histamine , immunoglobulin e , lipid peroxidation , chemistry , concanavalin a , pharmacology , endocrinology , immunology , medicine , biochemistry , antibody , biology , oxidative stress , in vitro
We previously showed that ganhuangenin (GHG) has beneficial antioxidative properties against lipid peroxidation in tissues of Sprague‐Dawley (SD) rats. In this study, the effect of GHG on immunoglobulin E (IgE), histamine and leukotriene B 4 (LTB 4 ) levels of cells isolated from SD rats was examined with regard to the manifestation of the type I allergic reaction. We showed that GHG inhibited the IgE production of mesenteric lymph node (MLN) lymphocytes. In the presence or absence of concanavalin A (ConA), the concentration used did not exert a toxic effect against MLN lymphocytes. Interestingly, the increase in the IgE content and lipid peroxidation induced by ConA was alleviated in the presence of GHG. Moreover, GHG also inhibited histamine release from the peritoneal exudate cells stimulated with a calcium ionophore, A23187, or with the histamine releaser, compound 48/80. In the case of LTB 4 , GHG markedly inhibited its release at a concentration of 100 μ M . Thus, it is concluded that GHG may block the common pathway for the release of histamine and LTB 4 , and that the IgE level is responsible for the lipid peroxidation induced by ConA. Copyright © 2002 John Wiley & Sons, Ltd.