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Antimalarial activity of extracts and alkaloids isolated from six plants used in traditional medicine in Mali and Sao Tome
Author(s) -
Ancolio C.,
Azas N.,
Mahiou V.,
Ollivier E.,
Di Giorgio C.,
Keita A.,
TimonDavid P.,
Balansard G.
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1025
Subject(s) - combretaceae , morinda , rubiaceae , traditional medicine , biology , pharmacognosy , apocynaceae , lythraceae , botany , medicine , biological activity , in vitro , biochemistry
Methanol and chloroform extracts were prepared from various parts of four plants collected in Mali: Guiera senegalensis (Gmel.) Combretaceae, Feretia apodanthera (Del.) Rubiaceae, Combretum micranthum (Don.) Combretaceae, Securidaca longepedunculata (Fres.) Polygalaceae and two plants collected in Sao Tome: Pycnanthus angolensis (Welw.) Myristicaceae and Morinda citrifolia (Benth.) Rubiaceae were assessed for their in vitro antimalarial activity and their cytotoxic effects on human monocytes (THP1 cells) by flow cytometry. The methanol extract of leaves of Feretia apodanthera and the chloroform extract of roots of Guiera senegalensis exhibited a pronounced antimalarial activity. Two alkaloids isolated from the active extract of Guiera senegalensis , harman and tetrahydroharman, showed antimalarial activity (IC 50 lower than 4 μg/mL) and displayed low toxicity against THP1. Moreover, the decrease of THP1 cells in S phase of the cell cycle, after treatment with harman and tetrahydroharman, was probably due to an inhibition of total protein synthesis. Copyright © 2002 John Wiley & Sons, Ltd.