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Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using ELISA
Author(s) -
Noda Kiyoshi,
Tanaka Kuniaki,
Yamada Akira,
Ogata Jyunko,
Tanaka Hiroyuki,
Shoyama Yukihiro
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1021
Subject(s) - monoclonal antibody , chlorella vulgaris , glycoprotein , strain (injury) , biological activity , in vitro , microbiology and biotechnology , biology , pharmacognosy , cell culture , protease , antibody , chemistry , biochemistry , immunology , enzyme , algae , anatomy , ecology , genetics
Abstract A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross‐reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross‐reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains. Copyright © 2002 John Wiley & Sons, Ltd.