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Transmission Microsphere‐Assisted Dark‐Field Microscopy
Author(s) -
Perrin Stephane,
Li Hongyu,
Badu Keshia,
Comparon Thomas,
Quaranta Giorgio,
Messaddeq Nadia,
Lemercier Nicolas,
Montgomery Paul,
Vonesch JeanLuc,
Lecler Sylvain
Publication year - 2019
Publication title -
physica status solidi (rrl) – rapid research letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.786
H-Index - 68
eISSN - 1862-6270
pISSN - 1862-6254
DOI - 10.1002/pssr.201800445
Subject(s) - dark field microscopy , diffraction , optical microscope , microsphere , materials science , microscopy , optics , microscope , glass microsphere , optoelectronics , nanotechnology , physics , scanning electron microscope , chemical engineering , engineering
Microsphere‐assisted microscopy allows the limit of the diffraction of light to overcome while being non‐invasive, full‐field, label‐free, and easy‐to‐implement. However, the observation of translucent samples remains difficult using a classical bright‐field illumination. In this work, a method is presented for the inspection of quasi‐transparent sub‐diffraction‐limited structures by using dark‐field illumination in the transmission mode. Glass‐imprint features, having a size of 250 nm, as well as fixed mouse brain cells have been visualized using the dark‐field microsphere‐assisted technique. The possibility to observe feature sizes up to 100 nm has been demonstrated in air using a 25‐µm‐diameter glass microsphere combined with an optical microscope, opening new possibilities for biological imaging.