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Adhesion of osteoblasts on chemically patterned nanocrystalline diamonds
Author(s) -
Kalbacova Marie,
Michalikova Lenka,
Baresova Veronika,
Kromka Alexander,
Rezek Bohuslav,
Kmoch Stanislav
Publication year - 2008
Publication title -
physica status solidi (b)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.51
H-Index - 109
eISSN - 1521-3951
pISSN - 0370-1972
DOI - 10.1002/pssb.200879579
Subject(s) - adhesion , nanotechnology , focal adhesion , cell adhesion , materials science , biosensor , drug delivery , fluorescence microscope , actin , diamond , biophysics , surface modification , chemistry , fluorescence , cell , composite material , biochemistry , physics , biology , quantum mechanics
The compatibility of engineered surfaces with tissues, cells and biological molecules is critical in development of medical diagnostics, biosensors, drug delivery systems, etc. Nanocrystalline diamond (NCD) films represent group of novel materials with superior electro‐optical, chemical, and mechanical properties for such devices. We studied adhesion and viability of human osteoblastic cells on NCD films with the aim to define optimal semiconductor interfaces for novel thin‐film biosensor. We cultured human osteoblasts (SAOS‐2) at different densities on chemically terminated NCD films with periodical O‐ and H‐terminated stripes of different width – 30, 100 and 200 µm and analysed cell cytoskeleton (actin filaments) and focal adhesions by fluorescence microscopy. We found that cell attachment is guided by the hydrophilic/hydrophobic patterns with preferential initial cellular adhesion on hydrophilic (O‐terminated) surface. Cell adhesion seems to be mediated by preferential culture medium proteins adsorption on the surface. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)