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Electrochemical Impedance Spectroscopy Using Interdigitated Gold–Polypyrrole Electrode Combination
Author(s) -
Kremers Tom,
Menzel Nora,
Freitag Fabian,
Laaf Dominic,
Heine Viktoria,
Elling Lothar,
Schnakenberg Uwe
Publication year - 2020
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201900827
Subject(s) - dielectric spectroscopy , polypyrrole , electrode , biosensor , materials science , horseradish peroxidase , polystyrene , reference electrode , analytical chemistry (journal) , electrochemistry , chemistry , nanotechnology , polymer , chromatography , organic chemistry , composite material , enzyme
A novel electrochemical impedance spectroscopy (EIS) sensor design, based on a standard interdigitated electrode arrangement in which the working electrode consists of gold and the combined counter and reference electrodes of polypyrrole doped with polystyrene sulfonate (PPy:PSS), is evaluated for biosensing applications. The performance is successfully proved by immobilization of a thiolated biotin as a self‐assembled monolayer (SAM), followed by streptavidin and a biotinylated horseradish peroxidase. It is shown that specific binding of biomolecules takes place only at the gold electrode. The binding activities are not influenced by the addition of small amounts of the nonionic surfactant Pluronic F‐68. The immobilization process is monitored online with EIS showing an excellent repeatability of the EIS signals in comparison with Au–Au electrode configuration even after electrode regeneration.