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Structure and Cytocompatibility of a Porous Biomimetic Material for Oral Tissue Wound Healing
Author(s) -
Craciunescu Oana,
GasparPintiliescu Alexandra,
Seciu AnaMaria,
Moldovan Lucia,
Zarnescu Otilia
Publication year - 2019
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201800638
Subject(s) - extracellular matrix , fibronectin , scanning electron microscope , wound healing , chondroitin sulfate , materials science , transmission electron microscopy , biomedical engineering , composite number , scaffold , hyaluronic acid , matrix (chemical analysis) , chemical engineering , chemistry , glycosaminoglycan , composite material , nanotechnology , anatomy , biochemistry , medicine , surgery , engineering
Composite materials containing natural biocompatible polymers, specific for the extracellular matrix present in the periodontal tissue, are prepared by mixing type I soluble collagen (COL) and fibronectin (FN) as fibrillar proteins and chondroitin sulfate (CS) as polysaccharidic component. The three‐dimensional porous structure is obtained by freeze‐drying process and it is analyzed by scanning electron microscopy (SEM). Specific techniques of immunohistochemistry and transmission electron microscopy (TEM) are used to investigate the distribution and interaction between polymeric components within the scaffold. Light and electron microscopy observations are also performed on solutions of CS and FN to reveal their structural and morphological properties. The cytocompatibility of composite material is tested in gingival fibroblasts and osteoblast cell cultures using quantitative assays of cell viability. The results demonstrate that COL‐CS‐FN composite material has optimal characteristics for further testing as device for oral tissue wound healing.