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A Simple Paper Based Microfluidic Electrochemical Biosensor for Point‐of‐Care Cholesterol Diagnostics
Author(s) -
Kaur Gurpreet,
Tomar Monika,
Gupta Vinay
Publication year - 2017
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201700468
Subject(s) - cholesterol oxidase , microfluidics , materials science , photoresist , electrode , biosensor , nanotechnology , photolithography , working electrode , filter paper , optoelectronics , electrochemistry , chromatography , chemistry , layer (electronics) , cholesterol , biochemistry
Paper based microfluidic devices have emerged as a simple and low cost platform for efficient clinical diagnostics. An effort has been made in the present work to develop a microfluidic cholesterol biosensor using paper based platform. Microfluidic channels have been fabricated by patterning filter paper (Whatman 1) using photolithography. SU 8 photoresist formed a hydrophobic barrier on the paper with microchannels of dimensions 1000 μm(w)×100 μm(t). A three electrode system was fabricated by simple mask printing of graphite as the working, as well as the counter electrodes, and silver as the reference electrode. The working electrode was modified using nickel oxide nanoparticles prepared using co‐precipitation method. Cholesterol oxidase enzyme is spotted on the test zone and chronoamperometric measurements are taken for different concentrations of cholesterol in the range of 0.12–10.23 mM, giving a quick and sensitive detection with a sensitivity of 24 µA mM −1 cm −2 .