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Optimization of the immobilization of bacterial spores on glass substrates with organosilanes
Author(s) -
Arreola Julio,
Mätzkow Malte,
Durán Marlena Palomar,
Greeff Anton,
Keusgen Michael,
Schöning Michael J.
Publication year - 2016
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201532914
Subject(s) - silanization , silane , sonication , biosensor , chemical engineering , materials science , curing (chemistry) , solvent , photopolymer , chemistry , chromatography , polymer chemistry , polymer , organic chemistry , composite material , nanotechnology , engineering , polymerization
Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween ® 80) and sonication. AFM image of silanization on a glass substrate with an organosilane (e.g., APTES).