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Studying the spatially resolved immobilisation of enzymes on a capacitive field‐effect structure by means of nano‐spotting
Author(s) -
Beging Stefan,
Leinhos Marcel,
Jablonski Melanie,
Poghossian Arshak,
Schöning Michael J.
Publication year - 2015
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201431891
Subject(s) - capacitive sensing , capacitance , dielectric spectroscopy , electrolyte , drop (telecommunication) , nano , materials science , biosensor , coating , optoelectronics , analytical chemistry (journal) , nanotechnology , spotting , chemistry , electrode , chromatography , optics , composite material , electrochemistry , electrical engineering , physics , engineering
Conventional methods for enzyme immobilisation onto sensor surfaces often use dip‐, drop‐ or spin‐coating techniques. In this study, a nano‐spotting technique has been investigated for a patterned, spatially resolved deposition of enzymes onto a capacitive field‐effect electrolyte–insulator–semiconductor (EIS) sensor and compared with the drop‐coating method. Therefore, four different sensor arrangements covered with immobilised penicillinase as a model enzyme have been studied: (i) The sensor surface fully drop‐coated, (ii) half drop‐coated, (iii) half nano‐spotted and (iv) fully nano‐spotted with penicillinase. The sensors have been electrochemically characterised in pH buffers and penicillin solutions by means of impedance‐spectroscopy, capacitance–voltage and constant‐capacitance methods.