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From 2D to 1D functionalization: Steps towards a carbon nanotube based biomembrane sensor for curvature sensitive proteins
Author(s) -
Ostermaier Frieder,
Scharfenberg Linda,
Schneider Kristian,
Hennig Stefan,
Ostermann Kai,
Posseckardt Juliane,
Rödel Gerhard,
Mertig Michael
Publication year - 2015
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201431723
Subject(s) - carbon nanotube , membrane curvature , lipid bilayer , surface modification , curvature , biological membrane , membrane , bilayer , materials science , nanotechnology , biophysics , fluorescence , chemistry , biochemistry , biology , optics , physics , geometry , mathematics
Membrane proteins are not homogeneously distributed throughout the membrane. One proposed localization mechanism is related to membrane curvature. In this work a sensor platform has been developed that uses carbon nanotubes to create curvature in a supported lipid bilayer. The specific binding of curvature sensitive proteins to the bent areas of the membrane is investigated by fluorescence microscopy. The setup will allow to study binding kinetics of curvature sensing motifs or to use such motifs for a one‐dimensional functionalization along the CNT. Fluorescence image of EGFP labeled curvature sensitive proteins that show linear alignment on a supported lipid bilayer above dielectrophoretically deposited single‐walled carbon nanotubes.