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Impact of carbon nanomaterials on the formation of multicellular spheroids by tumor cells
Author(s) -
Yakymchuk O. M.,
Perepelytsina O. M.,
Rud A. D.,
Kirian I. M.,
Sydorenko M. V.
Publication year - 2014
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201431358
Subject(s) - spheroid , nanomaterials , materials science , substrate (aquarium) , adhesion , fullerene , cell adhesion , biophysics , multicellular organism , carbon fibers , population , nanotechnology , cell culture , cell , chemistry , chemical engineering , in vitro , biology , composite material , biochemistry , organic chemistry , composite number , ecology , genetics , engineering , demography , sociology
This paper investigates the effect of different concentrations of nanostructured materials: fullerene‐like (C 60 ), onion‐like carbon (OLC) and ultradispersed diamonds (UDD) on the formation of multicellular spheroids. The chemical composition and purity of nanomaterials are controlled by Fourier transform infrared spectroscopy. The strength and direction of the impact of nanomaterials on the cell population was assessed using microphotography of tumor multicellular spheroids of breast adenocarcinoma cell line MCF‐7 in vitro and Pearson's correlation coefficient. The results demonstrated that UDD and OLC reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids of ∼3 × 10 −3 mm 3 in significant amount. The fullerenes reduced in the main cell adhesion to substrate that led to formation of cell aggregates of ∼5 × 10 −3 mm 3 . The results could be useful for achievement of the directed cell growth in three‐dimensional culture.