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Nutrient concentration‐sensitive microorganism‐based biosensor
Author(s) -
Werner Carl Frederik,
Groebel Simone,
Krumbe Christoph,
Wagner Torsten,
Selmer Thorsten,
Yoshinobu Tatsuo,
Baumann Marcus E. M.,
Keusgen Michael,
Schöning Michael J.
Publication year - 2012
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201100801
Subject(s) - biosensor , microorganism , nutrient , chemistry , escherichia coli , bacteria , extracellular , biochemistry , biology , genetics , organic chemistry , gene
Abstract This work presents a biosensor based on living cells immobilised on a light‐addressable potentiometric sensor (LAPS). The set‐up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme‐based field‐effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on‐chip differential set‐up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised.