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Diamond electrolyte solution gate FETs for DNA and protein sensors using DNA/RNA aptamers
Author(s) -
Kawarada H.,
Ruslinda A. R.
Publication year - 2011
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.201100503
Subject(s) - biomolecule , dna , biosensor , rna , diamond , aptamer , chemistry , nanotechnology , biophysics , materials science , microbiology and biotechnology , biochemistry , biology , gene , organic chemistry
For DNA and protein detection, we have analyzed DNA/RNA immobilized biosensors based on diamond field effect transistors (FETs), where several types of atomic or molecule termination have been realized on their surfaces. Amine termination and carboxyl termination necessary for biomolecule immobilization can be formed directly on the diamond surfaces resulting in the immobilization of short DNA/RNA within 5 nm from the surface. Owing to the high areal capacitance of a liquid electric double layer capacitor and a solid channel capacitor, the change in the surface charge caused by the hybridization of DNA/RNA and the protein binding to DNA/RNA aptamers can be efficiently detected. The charge of surface termination groups such as ${\rm NH}_{{\rm 3}}^{{\rm + }} $ , O − , COO − affects the suppression of nonspecific binding. The detection of biomolecules such as one‐base mismatch of DNA and proteins that is unaffected by nonspecific binding can be realized as a result.