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Synthetic diamond films as a platform material for label‐free protein sensors
Author(s) -
Bijnens Nathalie,
Vermeeren Veronique,
Daenen Michaël,
Grieten Lars,
Haenen Ken,
Wenmackers Sylvia,
Williams Oliver A.,
Ameloot Marcel,
vandeVen Martin,
Michiels Luc,
Wagner Patrick
Publication year - 2009
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.200880485
Subject(s) - nanocrystalline material , diamond , dielectric spectroscopy , electrical impedance , adsorption , c reactive protein , materials science , analytical chemistry (journal) , chemistry , optoelectronics , nanotechnology , chromatography , electrode , composite material , electrical engineering , medicine , electrochemistry , engineering , inflammation
Abstract In the framework of developing a fast and label‐free immunosensor for C‐reactive protein (CRP) detection, H‐terminated nanocrystalline diamond (NCD) was functionalised with anti‐CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real‐time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC‐labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 μM CRP were recognised with a reaction time of 30 minutes. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)