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Immobilisation of biomolecules for biosensors
Author(s) -
Barlen Benjamin,
Mazumdar Saikat Datta,
Keusgen Michael
Publication year - 2009
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.200880478
Subject(s) - biosensor , surface plasmon resonance , biomolecule , chemistry , nanotechnology , combinatorial chemistry , nanoparticle , biochemistry , materials science
Immobilisation of proteins, mainly enzymes and antibodies, is one of the most crucial steps in the development of biosensors. Unspecific methods as well as site‐directed strategies have been developed in order to obtain highest functionality and stability. Different methods for immobilisation of antibodies were tested to develop a surface plasmon resonance (SPR) biosensor for the detection of Salmonella . It turned out, that best results in terms of capturing and detection of Salmonella were achieved by immobilisation via hydrophobic interaction, using a sensor surface coated with C 18 ‐alkyl chains allowing integration of hydrophobic parts of the antibody. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)