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Penicillin detection with nanocrystalline‐diamond field‐effect sensor
Author(s) -
Abouzar Maryam H.,
Poghossian Arshak,
Razavi Arash,
Besmehn Astrid,
Bijnens Nathalie,
Williams Oliver A.,
Haenen Ken,
Wagner Patrick,
Schöning Michael J.
Publication year - 2008
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.200879713
Subject(s) - biosensor , diamond , detection limit , materials science , capacitance , capacitive sensing , linear range , penicillin , chemical vapor deposition , analytical chemistry (journal) , electrolyte , optoelectronics , nanotechnology , chemistry , electrode , chromatography , composite material , electrical engineering , biochemistry , engineering , antibiotics
Nanocrystalline‐diamond (NCD) films have been utilised for the detection of penicillin G for the first time. The developed penicillin‐sensitive biosensor consists of a field‐effect capacitive electrolyte‐diamond‐insulator‐semiconductor (EDIS) structure with an immobilised enzyme layer that covers the gate region of the sensor. Undoped NCD thin films of about 100 nm thickness were grown on a p‐Si‐SiO 2 (50 nm thermally grown SiO 2 ) structure by microwave plasma‐enhanced chemical vapour deposition. The enzyme penicillinase has been adsorptively immobilised directly onto the O‐terminated NCD surface. The EDIS biosensors have been characterised in buffer solutions with different content of penicillin G by means of capacitance–voltage and constant‐capacitance method. The developed penicillin biosensor possesses a low detection limit of 5 µM and a high sensitivity of 60–70 mV/decade in a wide linear range of 0.005–2.5 mM penicillin G concentration. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)