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UV LED arrays at 280 and 340 nm for spectroscopic biosensing
Author(s) -
Davitt K.,
Song Y.K.,
Patterson W.,
Nurmikko A. V.,
Ren Z.,
Sun Q.,
Han J.
Publication year - 2007
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.200674925
Subject(s) - biosensor , ultraviolet , polystyrene , materials science , optoelectronics , fluorescence , light emitting diode , diode , nanotechnology , optics , physics , polymer , composite material
We demonstrate the application of linear arrays of ultraviolet light emitting diodes (UV LEDs) at the wavelengths of 280 nm and 340 nm to the acquisition of fluorescence spectra from single particles in real‐time. A compact optical system and custom electronics are employed to produce a compact, standalone biosensor. The detection sensitivity of the UV LED system is established using the biofluorophores tryptophan and NADH, and the capability of the sensor to sort particles based on weak spectra is demonstrated using micron‐sized polystyrene latex (PSL) spheres. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)