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Porous silicon‐based potentiometric biosensor for triglycerides
Author(s) -
Setzu S.,
Salis S.,
Demontis V.,
Salis A.,
Monduzzi M.,
Mula Guido
Publication year - 2007
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.200674378
Subject(s) - potentiometric titration , biosensor , wafer , silicon , porous silicon , mesoporous material , matrix (chemical analysis) , chemistry , fabrication , materials science , chemical engineering , chromatography , nanotechnology , electrode , organic chemistry , engineering , medicine , alternative medicine , pathology , catalysis
In this paper we report on the fabrication and characterization of a potentiometric biosensor for the detection of triglycerides. This is constituted by a lipase immobilized on a mesoporous Si matrix. Prototypes, realized on 1 × 1 cm n + ‐type silicon wafers, show a very high enzymatic activity. Moreover the properties of these biosensors have been shown to be stable in a several months time interval, clearly showing their advantages with respect to traditional triglycerides detection systems. The Michaelis Menten curve is obtained to demonstrate the absence of diffusion problems. Potentiometric measurements are also shown. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)