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Cellular uptake of cell‐penetrating peptides p VEC and transportan in plants
Author(s) -
Chugh Archana,
Eudes François
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.937
Subject(s) - internalization , pinocytosis , protoplast , peptide , fluorescence microscope , cell penetrating peptide , biophysics , plant cell , endocytosis , cell , biology , microbiology and biotechnology , biochemistry , chemistry , fluorescence , gene , physics , quantum mechanics
Internalization of fluorescently labeled CPPs, p VEC, transportan and scrambled p VEC, in a range of plant cells was investigated. Cellular uptake of the peptides was found to be tissue dependent. p VEC and transportan were distinctly internalized in triticale mesophyll protoplasts, onion epidermal cells, leaf bases and root tips of seven‐day old triticale seedlings but showed negligible florescence in coleoptile and leaf tips as observed under a fluorescence microscope. Further, p VEC and transportan uptake studies were focused on mesophyll protoplasts as a system of investigation. In fluorimetric studies transportan showed 2.3 times higher cellular internalization than p VEC in protoplasts, whereas scrambled p VEC failed to show any significant fluorescence. Effect of various factors on cellular internalization of p VEC and transportan in protoplasts was also investigated. The cellular uptake of both the peptides was concentration dependent and nonsaturable. The cellular uptake of p VEC and transportan was enhanced at low temperature (4 °C). The presence of endocytic/macropinocytosis inhibitors did not reduce the cellular uptake of the peptides, suggesting direct cell penetration, receptor‐independent internalization of p VEC and transportan into the plant cells. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.