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A Bowman‐Birk trypsin inhibitor with antiproliferative activity from Hokkaido large black soybeans
Author(s) -
Ho Vincent S. M.,
NG T. B.
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.922
Subject(s) - trypsin , trypsin inhibitor , biochemistry , microbiology and biotechnology , enzyme inhibitor , fusarium oxysporum , peptide , biology , enzyme , chemistry , botany
A trypsin inhibitor, with an N ‐terminal sequence highly homologous to those of 8‐kDa Bowman‐Birk trypsin inhibitors, was isolated from the seeds of Hokkaido large black soybeans. The trypsin inhibitor was unadsorbed on SP‐Sepharose but adsorbed on DEAE‐cellulose and Mono Q. It inhibited proliferation in breast cancer (MCF‐7) cells and hepatoma (Hep G2) cells with an IC 50 of 35 and 140 µ M , respectively. The trypsin inhibitory activity of the inhibitor was completely preserved after exposure to temperatures up to 100 °C for 30 min and to the pH range 2–13 for the same duration. The trypsin inhibitor inhibited HIV‐1 reverse transcriptase with an IC 50 of 38 µ M , but was devoid of antifungal activity toward Fusarium oxysporum and Mycosphaerella arachidicola . Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.