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Fast conventional Fmoc solid‐phase peptide synthesis with HCTU
Author(s) -
Hood Christina A.,
Fuentes German,
Patel Hirendra,
Page Karen,
Menakuru Mahendra,
Park Jae H.
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.921
Subject(s) - reagent , chemistry , hexafluorophosphate , peptide , peptide synthesis , methylene , combinatorial chemistry , coupling reaction , solid phase synthesis , chromatography , organic chemistry , ionic liquid , biochemistry , catalysis
Abstract 1 H ‐Benzotriazolium 1‐[ bis (dimethyl‐amino)methylene]‐5‐chloro‐hexafluorophosphate (1‐),3‐oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP‐6, 65–74 ACP, oxytocin, G‐LHRH, C ‐peptide, hAmylin 1–37 , and β‐amyloid 1–42 ) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid‐phase peptide synthesis. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.