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Solid‐phase peptide synthesis using nanoparticulate amino acids in water
Author(s) -
Hojo Keiko,
Ichikawa Hideki,
Maeda Mitsuko,
Kida Shinya,
Fukumori Yoshinobu,
Kawasaki Koichi
Publication year - 2007
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.874
Subject(s) - amino acid , peptide , chemistry , peptide synthesis , phase (matter) , solid phase synthesis , combinatorial chemistry , biochemistry , organic chemistry
Solid‐phase peptide synthesis has many advantages compared with solution peptide synthesis. However, this procedure requires a large amount of organic solvents. Since safe organic solvent waste disposal is an important environmental problem, a technology based on coupling reaction of suspended nanoparticle reactants in water was studied. Fmoc‐amino acids are used widely, but most of them show low solubility in water. We prepared well‐dispersible Fmoc‐amino acid nanoparticles in water by pulverization using a planetary ball mill in the presence of poly(ethylene glycol). Leu‐enkephalin amide was prepared successfully using the nanoparticulate Fmoc‐amino acid on a poly(ethylene glycol)‐grafted Rink amide resin in water. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.