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The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine‐knot motif
Author(s) -
Fujitani Naoki,
Kouno Takahide,
Nakahara Taku,
Takaya Kenji,
Osaki Tsukasa,
Kawabata Shunichiro,
Mizuguchi Mineyuki,
Aizawa Tomoyasu,
Demura Makoto,
Nishimura ShinIchiro,
Kawano Keiichi
Publication year - 2007
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.846
Subject(s) - peptide , antiparallel (mathematics) , structural similarity , horseshoe crab , chemistry , stereochemistry , antimicrobial , biochemistry , biology , physics , quantum mechanics , magnetic field , paleontology , organic chemistry
Tachystatin B is an antimicrobial and a chitin‐binding peptide isolated from the Japanese horseshoe crab ( Tachypleus tridentatus ) consisting of two isopeptides called tachystatin B1 and B2. We have determined their solution structures using NMR experiments and distance geometry calculations. The 20 best converged structures of tachystatin B1 and B2 exhibited root mean square deviations of 0.46 and 0.49 Å, respectively, for the backbone atoms in Cys 4 ‐Arg 40 . Both structures have identical conformations, and they contain a short antiparallel β‐sheet with an inhibitory cystine‐knot (ICK) motif that is distributed widely in the antagonists for voltage‐gated ion channels, although tachystatin B does not have neurotoxic activity. The structural homology search provided several peptides with structures similar to that of tachystatin B. However, most of them have the advanced functions such as insecticidal activity, suggesting that tachystatin B may be a kind of ancestor of antimicrobial peptide in the molecular evolutionary history. Tachystatin B also displays a significant structural similarity to tachystatin A, which is member of the tachystatin family. The structural comparison of both tachystatins indicated that Tyr 14 and Arg 17 in the long loop between the first and second strands might be the essential residues for binding to chitin. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.