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Rapid solid‐phase peptide synthesis using thermal and controlled microwave irradiation
Author(s) -
Bacsa Bernadett,
Desai Bimbisar,
Dibó GáBor,
Kappe C. Oliver
Publication year - 2006
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.771
Subject(s) - microwave irradiation , phase (matter) , solid phase synthesis , peptide , microwave , irradiation , peptide synthesis , chemistry , combinatorial chemistry , radiochemistry , materials science , biochemistry , organic chemistry , physics , engineering , nuclear physics , telecommunications , catalysis
Abstract A rapid and efficient microwave‐assisted solid‐phase synthesis method is described for the preparation of the nonapeptide WDTVRISFK, using conventional Fmoc/Bu t orthogonal protection strategy. The synthesis protocol is based on the use of cycles of pulsed microwave irradiation with intermittent cooling of the reaction during the removal of the Fmoc protecting group and during the coupling. The desired nonapeptide was obtained in highest yield and purity by employing MicroKan technology. The chemical reactions were carried out in a single‐mode microwave reactor, equipped with a fiber‐optic probe to monitor the reaction temperature continuously. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.