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Design of novel bicyclic analogues derived from a potent oxytocin antagonist
Author(s) -
Flouret George,
Chaloin Olivier,
Borovickova Lenka,
Slaninová Jirina
Publication year - 2006
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.742
Subject(s) - uterotonic , antagonist , chemistry , ethylenediamine , stereochemistry , oxytocin , potency , bicyclic molecule , receptor , medicine , biochemistry , in vitro , inorganic chemistry
Eleven new analogues were synthesized by modification of the potent oxytocin antagonist (OTA) [(S)Pmp 1 , D ‐Trp 2 , Pen 6 , Arg 8 ]‐Oxytocin, or PA (parent antagonist), in which (S)Pmp = β,β‐(3‐thiapentamethylene)‐β‐mercapto‐propionic acid. By internal acylation of Lys, Orn, L ‐1,4‐diaminobutyric acid (Dab), L ‐1,3‐diaminopropionic acid (Dap) at position 4 with the C ‐terminal Gly of the peptide tail, we prepared cyclo‐(4–9)‐[Lys 4 , Gly 9 ]‐PA (pA 2 = 8.77 ± 0.27), 1, and cyclo‐(4–9)‐[Orn 4 , Gly 9 ]‐PA (pA 2 = 8.81 ± 0.25), 3, which are equipotent with PA (pA 2 = 8.68 ± 0.18) in the rat uterotonic assay and cyclo‐(4–9)‐[Dab 4 , Gly 9 ]‐PA, 4, cyclo‐(4–9)‐[Dap 4 , Gly 9 ]‐PA, 5, and cyclo‐(4–9)‐[Pmp 1 , Lys 4 , Gly 9 ]‐PA, 2, which were weaker OTAs. Neither 1 nor 3 had activity as agonists or antagonists in the antidiuretic assay. In the pressor assay, both analogues 1 and 3, with pA 2 = 7.05 ± 0.10 and pA 2 = 6.77 ± 0.12, respectively, are somewhat weaker antagonists than PA (pA 2 = 7.47 ± 0.35) showing significant gain in specificity. The [desamido 9 ] PA‐ethylenediamine monoamide, 6, and the dimer ([desamido 9 ]‐PA) 2 ethylenediamine diamide, 7, had lower potency in the uterotonic assay than PA. Additionally, we synthesized cyclo‐(1–5)‐[(HN)Pmp 1 , Asp 5 ]‐PA, 8, inactive in all tests, which suggests that the intact Asn 5 side chain may be critical in the interaction of the OTAs with the oxytocin (OT) receptor. Similarly, cyclo‐(5–9)‐[Dap 5 , Gly 9 ]‐PA, 9, had very low uterotonic potency. Two derivatives of PA truncated from the C ‐terminus were internally cyclized to Lys 4 , giving rise to cyclo‐(4–8)‐desGly‐ $\hbox{NH}_{2}^{9}$ [Lys 4 , Arg 8 ]‐PA, 10 (pA 2 = 8.35 ± 0.20), which maintains the high potency of PA and has no activity in the rat antidiuretic assay, and in the rat pressor assay it is about ten times weaker (pA2 = 6.41 ± 0.15) than PA (pA2 = 7.47 ± 0.35), thus showing gains in specificity, and to cyclo‐(4–7)‐desArg‐Gly‐ $\hbox{NH}_{2}^{8-9}$ [Lys 4 , Pro 7 )‐PA, 11, which has much weaker potency than PA. Synthesis of cyclo‐(4–6)‐desPro‐Arg‐Gly‐ $\hbox{NH}_{2}^{7-9}$ [Lys 4 ]‐PA failed. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.