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Synthetic, site‐specific biotinylated analogs of human MCP‐1
Author(s) -
Kruszynski Marian,
Tsui Ping,
Stowell Nicole,
Luo Jinquan,
Nemeth Jennifer F.,
Das Anuk M.,
Sweet Raymond,
Heavner George A.
Publication year - 2006
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.734
Subject(s) - biotinylation , antibody , phage display , panning (audio) , peptide , chemistry , peptide library , monocyte , microbiology and biotechnology , homogeneous , computational biology , biochemistry , medicine , immunology , biology , peptide sequence , gene , physics , thermodynamics , paleontology , zoom , lens (geology)
Human monocyte chemoattractant protein 1 (MCP‐1, CCL2) is a 8.6‐kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP‐1, we synthesized site‐specific, biotinylated human MCP‐1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site‐specific biotinylated analogs were homogeneous and retained full activity. Copyright © 2005 European Peptide Society and John Wiley & Sons, Ltd.