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Labeling of proteins and oligopeptides with luminescent lanthanide(III) chelates
Author(s) -
Jaakkola Lassi,
Peuralahti Jari,
Hakala Harri,
Mukkala Velimatti,
Hurskainen Pertti,
Hovinen Jari
Publication year - 2006
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.697
Subject(s) - lanthanide , europium , samarium , chelation , oligopeptide , chemistry , combinatorial chemistry , peptide , peptide synthesis , terpyridine , luminescence , inorganic chemistry , organic chemistry , materials science , biochemistry , ion , optoelectronics , metal
Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine‐based europium(III) chelate designed for solution phase protein labeling is demonstrated. Copyright © 2005 European Peptide Society and John Wiley & Sons, Ltd.