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Nonconventional Protease Catalysis in Frozen Aqueous Solutions
Author(s) -
Hänsler Marion,
Jakubke HansDieter
Publication year - 1996
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.65
Subject(s) - proteases , chemistry , peptide , protease , enzyme catalysis , cysteine , peptide synthesis , catalysis , enzyme , aqueous solution , combinatorial chemistry , serine protease , yield (engineering) , serine , organic chemistry , biochemistry , materials science , metallurgy
During the past decade proteases have been widely used as catalysts in peptide synthesis. Unfortunately, they are not ideal ligases. Enzymatic peptide synthesis in frozen aqueous systems has been developed as an approach towards the suppression of competitive reactions. This paper summarizes reports concerning the behaviour of non‐enzymatic as well as of enzyme‐catalysed reactions when the reaction mixture is frozen. The advantages of freezing the reaction mixture in serine and cysteine protease‐catalysed peptide synthesis, the influence of modified reaction conditions and the possible reasons for the yield‐increasing effect of freezing are discussed.