Premium
Isolation of allicepin, a novel antifungal peptide from onion ( Allium cepa ) bulbs
Author(s) -
Wang H. X.,
Ng T. B.
Publication year - 2004
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.509
Subject(s) - allium , size exclusion chromatography , botrytis cinerea , fusarium oxysporum , chemistry , chromatography , peptide , gel electrophoresis , mycelium , mycosphaerella , polyacrylamide gel electrophoresis , biochemistry , biology , botany , enzyme
From the bulbs of the onion Allium cepa , a novel antifungal peptide distinct from the antimicrobial peptide previously reported from onion seeds was isolated. The antifungal peptide, designated allicepin, was purified with a procedure that involved aqueous extraction, ion exchange chromatography on DEAE‐cellulose, affinity chromatography on Affi‐gel blue gel and FPLC‐gel filtration on Superdex 75. Allicepin was unadsorbed on DEAE‐cellulose and adsorbed on Affi‐gel blue gel. The molecular weight of allicepin was estimated to be 10 K by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and gel filtration on Superdex 75. Allicepin exerted an inhibitory activity on mycelial growth in several fungal species including Botrytis cinerea , Fusarium oxysporum , Mycosphaerella arachidicola and Physalospora piricola. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.