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Biosynthetic uniform 13 C, 15 N‐labelling of zervamicin IIB. Complete 13 C and 15 N NMR assignment
Author(s) -
Ovchinnikova Tatyana V.,
Shenkarev Zakhar O.,
Yakimenko Zoya A.,
Svishcheva Natalia V.,
Tagaev Andrey A.,
Skladnev Dmitry A.,
Arseniev Alexander S.
Publication year - 2003
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.499
Subject(s) - labelling , physics , chemistry , biochemistry
Zervamicin IIB is a member of the α‐aminoisobutyric acid containing peptaibol antibiotics. A new procedure for the biosynthetic preparation of the uniformly 13 C‐ and 15 N‐enriched peptaibol is described. This compound was isolated from the biomass of the fungus‐producer Emericellopsis salmosynnemata strain 336 IMI 58330 obtained upon cultivation in the totally 13 C, 15 N‐labelled complete medium. To prepare such a medium the autolysed biomass and the exopolysaccharides of the obligate methylotrophic bacterium Methylobacillus flagellatus KT were used. This microorganism was grown in totally 13 C, 15 N‐labelled minimal medium containing 13 C‐methanol and 15 N‐ammonium chloride as the only carbon and nitrogen sources. Preliminary NMR spectroscopic analysis indicated a high extent of isotope incorporation (>90%) and led to the complete 13 C‐ and 15 N‐NMR assignment including the stereospecific assignment of Aib residues methyl groups. The observed pattern of the structurally important secondary chemical shifts of 1 H α , 13 CO and 13 C α agrees well with the previously determined structure of zervamicin IIB in methanol solution. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.