Poster presentations: Topic E | Zendy

Angus R. Brown | Zendy; Maryanne Covington | Zendy; Robert Newton | Zendy; R. Ramage | Zendy; Patricia Welch | Zendy

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Poster presentations: Topic E

Author(s) -

Angus R. Brown,

Maryanne Covington,

Robert Newton,

R. Ramage,

Patricia Welch

Publication year - 1996

Publication title -

journal of peptide science

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 66

eISSN - 1099-1387

pISSN - 1075-2617

DOI - 10.1002/psc.46

Subject(s) - monocyte , chemotaxis , chemistry , total synthesis , biochemistry , stereochemistry , biology , immunology , receptor

The affinity-based N (alpha)-amino protecting group tetrabenzo[a,c,g,i]fluorenyl-17 methoxycarbonyl (Tbfmoc) has been utilized as a hydrophobic probe to allow the simple, quick and highly effective isolation of a 76 residue cysteine-containing protein (MCP-1). The base-labile Tbfmoc group can be removed under very mild conditions, which preserve the thiol-containing protein in the reduced state. Oxidative folding was then used to furnish the biologically active beta-chemokine MCP-1.

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