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Poster presentations: Topic E
Author(s) -
Angus R. Brown,
Maryanne Covington,
Robert Newton,
R. Ramage,
Patricia Welch
Publication year - 1996
Publication title -
journal of peptide science
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.46
Subject(s) - monocyte , chemotaxis , chemistry , total synthesis , biochemistry , stereochemistry , biology , immunology , receptor
The affinity-based N (alpha)-amino protecting group tetrabenzo[a,c,g,i]fluorenyl-17 methoxycarbonyl (Tbfmoc) has been utilized as a hydrophobic probe to allow the simple, quick and highly effective isolation of a 76 residue cysteine-containing protein (MCP-1). The base-labile Tbfmoc group can be removed under very mild conditions, which preserve the thiol-containing protein in the reduced state. Oxidative folding was then used to furnish the biologically active beta-chemokine MCP-1.