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A Novel Peptide with ribonuclease and translation‐inhibitory activities from fruiting bodies of the oyster mushroom Pleurotus ostreatus
Author(s) -
Ye X. Y.,
Ng T. B.
Publication year - 2002
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.382
Subject(s) - pleurotus ostreatus , oyster , mushroom , ribonuclease , chemistry , pleurotus , biology , botany , biochemistry , fishery , rna , gene
Abstract From the fresh fruiting bodies of the oyster mushroom a peptide with a molecular weight of 9 kDa and demonstrating a novel N ‐terminal sequence GPCYLVAFYESSGRR was isolated. The isolation procedure involved ion exchange chromatography on CM‐Sepharose and Mono S. The peptide was adsorbed on both types of chromatographic media. The peptide demonstrated a ribonuclease activity of 650 U/mg toward yeast transfer RNA. It inhibited cell‐free translation in a rabbit reticulocyte lysate system with an IC 50 of 15 n M . Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.