A colorimetric enzyme‐linked on‐bead assay for identification of synthetic substrates of protein tyrosine kinases | Zendy

Martin Scott E. | Zendy; Peterson Blake R. | Zendy

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A colorimetric enzyme‐linked on‐bead assay for identification of synthetic substrates of protein tyrosine kinases

Author(s) -

Martin Scott E.,

Peterson Blake R.

Publication year - 2002

Publication title -

journal of peptide science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 66

eISSN - 1099-1387

pISSN - 1075-2617

DOI - 10.1002/psc.376

Subject(s) - enzyme , biochemistry , chemistry , tyrosine kinase , kinase , protein tyrosine phosphatase , signal transduction

Protein tyrosine kinases play key roles in the progression of numerous human diseases including several types of cancers. We report here a simple colorimetric assay for tyrosine kinase activity employing synthetic peptide substrates prepared on Tentagel synthesis beads. Phosphorylation of compounds on beads was detected with an antiphosphotyrosine antibody complexed with a secondary antibody–alkaline phosphatase conjugate. This assay may prove useful for the identification and characterization of synthetic substrates of this important class of enzymes. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

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