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A colorimetric enzyme‐linked on‐bead assay for identification of synthetic substrates of protein tyrosine kinases
Author(s) -
Martin Scott E.,
Peterson Blake R.
Publication year - 2002
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.376
Subject(s) - enzyme , biochemistry , chemistry , tyrosine kinase , kinase , protein tyrosine phosphatase , signal transduction
Abstract Protein tyrosine kinases play key roles in the progression of numerous human diseases including several types of cancers. We report here a simple colorimetric assay for tyrosine kinase activity employing synthetic peptide substrates prepared on Tentagel synthesis beads. Phosphorylation of compounds on beads was detected with an antiphosphotyrosine antibody complexed with a secondary antibody–alkaline phosphatase conjugate. This assay may prove useful for the identification and characterization of synthetic substrates of this important class of enzymes. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.