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Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
Author(s) -
Govaerts Cindy,
Orwa Jennifer,
Schepdael Ann Van,
Roets Eugène,
Hoogmartens Jos
Publication year - 2002
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.367
Subject(s) - chromatography , chemistry , electrospray ionization , mass spectrometry , ion trap , electrospray , tandem mass spectrometry , top down proteomics , sample preparation in mass spectrometry , liquid chromatography–mass spectrometry , extractive electrospray ionization , protein mass spectrometry
A selective reversed phase liquid chromatography/mass spectrometry (LC/MS n ) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these polypeptide antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on‐line LC/MS n capability. The main advantage of this hyphenated LC/MS n technique is the characterization of novel related substances without time‐consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.