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Gel‐phase synthesis of hydrophobic [Thr 14 ] [Thr 19 ] Galanin (1–19) fragment on a high capacity flexible crosslinked polystyrene support
Author(s) -
Krishna Kumar I. M.,
Mathew Beena
Publication year - 2001
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.357
Subject(s) - polystyrene , copolymer , chemistry , yield (engineering) , polymer chemistry , styrene , suspension polymerization , polymerization , high performance liquid chromatography , chromatography , organic chemistry , polymer , materials science , metallurgy
A hydrophobic analogue of human galanin (1–19) fragment has been synthesized using Boc/Bzl tactics to demonstrate the synthetic utility of the flexible crosslinked polystyrene support prepared by the suspension polymerization of styrene and 1,4‐butanediol dimethacrylate. The copolymer was chloromethylated to 2.36 mmol Cl/g. The functionalized resin was found to possess all the physicochemical properties similar to Merrifield resin. The free peptide was obtained in high yield and purity as judged by RP‐HPLC and characterized by amino acid analysis and ESI‐MS. Copyright © 2000 European Peptide Society and John Wiley & Sons, Ltd.