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Anti‐colon cancer activity tracking isolation of peptide from ginseng leaves and potential mechanisms evaluation in vitro and in vivo
Author(s) -
Liu Zhuo,
Liu Xiaolei,
Li Wei,
Luo Qiang,
Liu Jie,
Wang Dongxin
Publication year - 2021
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.3297
Subject(s) - in vivo , apoptosis , ginseng , colorectal cancer , in vitro , caspase , cancer research , cancer , cancer cell , chemistry , pharmacology , biology , medicine , programmed cell death , pathology , biochemistry , alternative medicine , microbiology and biotechnology
The ginseng has been used for over hundred years, in the belief of promoting longevity. However, the anticancer activity of ginseng leaf peptide (GP) has been never explored. In current study, we isolated the GPs and explored the anti‐colon cancer activity in vitro and in vivo. MTT results showed that the GP‐1 (GP‐1~FKEHGY) performed most antiproliferative activity against colon cancer CT‐26 cells with an IC 50 of 86.4 ± 9.46 μM (48 h). Further study indicated that GP‐1 activated the caspases, regulated the p53/murine double minute 2 (MDM2) state, and induced the CT‐26 cells apoptosis in a mitochondrial pathway. Meanwhile, the GP‐1 arrested the CT‐26 cells in G0/G1 phase accompanied with cyclin expression regulation. In addition, GP‐1 significantly suppressed the tumor growth and induced the tumor cells apoptosis in vivo. Notably, the GP‐1 would be a potential anti‐colon cancer candidate.