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Investigation of intestinal transportation of peptide‐displaying bacteriophage particles using phage display method
Author(s) -
Kanaani Hakimeh,
Azarmi Yadollah,
Dastmalchi Siavoush,
Zarei Omid,
HamzehMivehroud Maryam
Publication year - 2021
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.3292
Subject(s) - biopanning , bacteriophage , phage display , peptide , nanocarriers , peptide library , chemistry , amino acid , computational biology , drug delivery , microbiology and biotechnology , biology , peptide sequence , biochemistry , organic chemistry , escherichia coli , gene
To investigate whether peptide sequences with specific translocation across the gastrointestinal barrier can be identified as drug delivery vehicles, in vivo phage display was conducted. For this purpose, a random library of 12‐mer peptides displayed on M13 bacteriophage was orally administered to mice followed by recovery of the phage particles from the blood samples after three consecutive biopanning rounds. The obtained peptide sequences were analyzed using bioinformatics tools and software. The results demonstrated that M13 bacteriophage bearing peptides translocate nonspecifically across the mice intestinal mucosal barrier deduced from random distribution of amino acids in different positions of the identified peptide sequences. The most probable reason for entering the phage particles into systemic circulation after oral administration of the peptide library can be related to the nanoscale nature of their structures which provides a satisfying platform for the purpose of designing nanocarriers in pharmaceutical applications.