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Configuration and racemization determination of cysteine residues in peptides by chiral derivatization and HPLC: application to oxytocin peptides
Author(s) -
Szabó Sándor,
Szókán Gyula,
Khlafulla Abdulrahim M.,
Almás Márta,
Kiss Csaba,
Rill Attila,
Schön István
Publication year - 2001
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.325
Subject(s) - racemization , derivatization , chemistry , oxytocin , cysteine , high performance liquid chromatography , chromatography , organic chemistry , enzyme , biology , endocrinology
An improved RP‐HPLC method was developed for the determination of the configuration and stereochemical purity of cysteine residues in peptides. The method consists of oxidation of cysteine and cystine residues to cysteic acid, followed by hydrolysis and pre‐column chiral derivatization with Val‐Marfey's reagent. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd.