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Antimicrobial function of short amidated peptide fragments from the tick‐derived OsDef2 defensin
Author(s) -
Ismail Naadhira O.,
Odendaal Clerisa,
Serem June C.,
Strömstedt Adam A.,
Bester Megan J.,
Sayed Yasien,
Neitz Albert W.H.,
Gaspar Anabella R.M.
Publication year - 2019
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.3223
Subject(s) - defensin , peptide , bacteria , chemistry , antibacterial activity , escherichia coli , biochemistry , peptide sequence , antimicrobial peptides , haemolysis , gram negative bacteria , antimicrobial , microbiology and biotechnology , staphylococcus aureus , liposome , melittin , biology , genetics , gene , immunology
Previously Os, a 22 amino acid sequence of a defensin from the soft tick Ornithodoros savignyi , was found to kill Gram‐positive and Gram‐negative bacteria at low micromolar concentrations. In this study, we evaluated synthetic peptide analogues of Os for antibacterial activity with an aim to identify minimalized active peptide sequences and in so doing obtain a better understanding of the structural requirements for activity. Out of eight partially overlapping sequences of 10 to 12 residues, only Os(3–12) and Os(11–22) exhibit activity when screened against Gram‐positive and Gram‐negative bacteria. Carboxyamidation of both peptides increased membrane‐mediated activity, although carboxyamidation of Os(11–22) negatively impacted on activity against Staphylococcus aureus . The amidated peptides, Os(3–12)NH 2 and Os(11–22)NH 2 , have minimum bactericidal concentrations of 3.3 μM against Escherichia coli . Killing was reached within 10 minutes for Os(3–12)NH 2 and only during the second hour for Os(11–22)NH 2 . In an E. coli membrane liposome system, both Os and Os(3–12)NH 2 were identified as membrane disrupting while Os(11–22)NH 2 was less active, indicating that in addition to membrane permeabilization, other targets may be involved in bacterial killing. In contrast to Os, the membrane disruptive effect of Os(3–12)NH 2 did not diminish in the presence of salt. Neither Os nor its amidated derivatives caused human erythrocyte haemolysis. The contrasting killing kinetics and effects of amidation together with structural and liposome leakage data suggest that the 3–12 fragment relies on a membrane disruptive mechanism while the 11–22 fragment involves additional target mechanisms. The salt‐resistant potency of Os(3–12)NH 2 identifies it as a promising candidate for further development.