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Development of poly(lactide‐co‐glycolide) nanoparticles functionalized with a mitochondria penetrating peptide
Author(s) -
Selmin Francesca,
Magri Giulia,
Gennari Chiara G.M.,
Marchianò Silvia,
Ferri Nicola,
Pellegrino Sara
Publication year - 2017
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2952
Subject(s) - plga , peptide , circular dichroism , mitochondrion , conjugate , chemistry , cytotoxicity , mtt assay , nanoparticle , biophysics , cell penetrating peptide , combinatorial chemistry , stereochemistry , biochemistry , cell , nanotechnology , materials science , in vitro , biology , mathematical analysis , mathematics
The development of mitochondria‐targeting cell permeable vectors represents a promising therapeutic approach for several diseases, such as cancer and oxidative pathologies. Nevertheless, access to mitochondria can be difficult. A new hybrid material composed by poly(lactide‐co‐glycolide) (PLGA) functionalized with a 6‐mer mitochondria penetrating peptide (MPP), consisting in alternating arginine and unnatural cyclohexylalanine, was developed. Circular dichroism, FT‐IR and DSC studies indicated that the conjugation of the peptide with the polymer led to the obtainment of a more rigid material with respect to both PLGA and MPP as such. In particular, a conformational rearrangement to a helical structure was observed for MPP. MPP – PLGA conjugates were used for the preparation of nanoparticles that showed no cytotoxicity in MTT assay, suggesting their putative use for future studies on mitochondria targeting. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.