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Gadolinium containing telechelic PEG‐polymers end‐capped by di‐phenylalanine motives as potential supramolecular MRI contrast agents
Author(s) -
Diaferia Carlo,
Gianolio Eliana,
Accardo Antonella,
Morelli Giancarlo
Publication year - 2017
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2942
Subject(s) - chemistry , dynamic light scattering , circular dichroism , antiparallel (mathematics) , moiety , gadolinium , peptide , supramolecular chemistry , peg ratio , beta sheet , polymer , fourier transform infrared spectroscopy , self healing hydrogels , polymer chemistry , crystallography , stereochemistry , nanoparticle , organic chemistry , chemical engineering , materials science , nanotechnology , biochemistry , crystal structure , physics , finance , quantum mechanics , magnetic field , engineering , economics
Telechelic PEG‐polymers end‐capped by diphenylalanine (FF) motives and containing a DOTA‐Gd complex, bound on a lysine side chain at the centre of peptide moiety, are studied for their assembling properties and for the relaxometric behavior. The observed variations in terms of relaxivity are correlated to the assembling properties of the aggregates by using several techniques: fluorescence, Circular Dichroism (CD) and Fourier Transform Infrared (FTIR) for aggregation tendency and secondary structure determination; Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) for morphological definition. Self‐aggregation in water solution of the peptide conjugates, due to interaction of the phenylalanine frameworks, starts at concentration around 1 mg/ml with a first evidence of the coexistence of fibrillary networks and hydrogels at 10 mg/ml. Definitive presence of well‐structured fibrillary networks, dominated by an antiparallel β ‐sheet arrangement, occurs at 50 mg/ml. At the latter concentration relaxivity values measured at 20 MHz and 298 K, are around 11 mM −1 s −1 , in line with a possible use of the these aggregates as MRI contrast agents. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.