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Peptide purification using the chemoselective reaction between N‐ (methoxy)glycine and isothiocyanato‐functionalized resin
Author(s) -
Hara Toshiaki,
Tainosyo Akira,
Kawakami Toru,
Aimoto Saburo,
Murata Michio
Publication year - 2016
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2888
Subject(s) - edman degradation , trifluoroacetic acid , peptide , chemistry , glycine , residue (chemistry) , amino acid residue , solubility , amino acid , peptide synthesis , chromatography , combinatorial chemistry , peptide sequence , organic chemistry , biochemistry , gene
An efficient peptide purification strategy is established, comprising the selective reaction of an N‐terminal N ‐(methoxy)glycine residue of the peptide and isothiocyanato‐functionalized resins, and subsequent Edman degradation. These reactions take place in acidic media; in particular, the Edman degradation proceeds smoothly in media containing more than 50% trifluoroacetic acid (v/v). These acidic conditions offer increased solubility, making them advantageous for the purification of hydrophobic and aggregation‐prone peptides. The effectiveness of this method, together with scope and limitations, is demonstrated using model peptides and the practical purification of the loop region of the human dopamine D2 receptor long isoform (residues 240–272). Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.