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Shifting gear in antimicrobial and anticancer peptides biophysical studies: from vesicles to cells
Author(s) -
Freire João M.,
Gaspar Diana,
Veiga Ana Salomé,
Castanho Miguel A. R. B.
Publication year - 2015
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2741
Subject(s) - antimicrobial peptides , vesicle , antimicrobial , mechanism of action , peptide , chemistry , cancer cell , computational biology , biochemistry , biophysics , membrane , biology , cancer , in vitro , organic chemistry , genetics
Despite the intensive study on the mechanism of action of membrane‐active molecules such as antimicrobial and anticancer peptides, most of the biophysical work has been performed using artificial model systems, mainly lipid vesicles. The use of these systems allows full control of the experimental parameters, and to obtain molecular‐level detail on the action of peptides, the correlation with biological action is intangible. Recently, several biophysical methodologies have been translated to studies using bacterial and cancer cells. Here, we review biophysical studies on the mechanism of action of antimicrobial and anticancer peptides performed directly on cells. The data in these studies allow to correlate vesicle‐based and cell‐based studies and fill the vesicle‐cell interdisciplinary gap. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd.