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Functional properties of a novel hybrid antimicrobial peptide NS: potent antitumor activity and efficient plasmid delivery
Author(s) -
Zhang Yun,
Song Jingjing,
Zhang Wei,
Liang Ranran,
Ma Yinyun,
Zhang Li,
Wei Xiaojin,
Ni Jingman,
Wang Rui
Publication year - 2014
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2667
Subject(s) - antimicrobial , peptide , cytotoxicity , antimicrobial peptides , transfection , moiety , chemistry , plasmid , multiple drug resistance , mechanism of action , cell penetrating peptide , in vitro , biochemistry , dna , antibiotics , gene , stereochemistry , organic chemistry
Antimicrobial peptides have been widely recognized as potential candidates for treating tumor, especially for defending against multidrug‐resistant cells. Previously, based on the structure of substance P, we have designed a novel class of hybrid antimicrobial peptide NS, which possesses potent antimicrobial activity against a broad spectrum of bacterial pathogens. In this study, we evaluated its cytotoxicity to tumor cells and studied the possible mechanism of action. We showed that NS could efficiently kill tumor cells by rapidly disrupting the tumor cell membrane and inhibiting the DNA synthesis. In addition, we also found that NS could efficiently deliver plasmids into cells and exhibit high transfection efficiency after the introduction of a stearyl moiety to its N‐terminus, like many reported cell‐penetrating peptides. Taken together, this study revealed the potential multiple functions of NS, providing fundamental support for further therapeutic application as potential antitumor agent. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.