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An apolipoprotein E mimetic peptide with activities against multidrug‐resistant bacteria and immunomodulatory effects
Author(s) -
Wang Chuanqing,
Yang Changsheng,
Yang Yi,
Pan Fu,
He Leiyan,
Wang Aimin
Publication year - 2013
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2570
Subject(s) - multiple drug resistance , peptide , pharmacology , chemistry , bacteria , microbiology and biotechnology , biology , biochemistry , antibiotics , genetics
Apolipoprotein E (apoE) mimetic peptides derived from the low‐density lipoprotein receptor‐binding region of apoE with both activities against multidrug‐resistant bacteria and immunomodulatory effects have not previously been reported. We identified an apoE mimetic peptide analogue of the receptor‐binding region of apoE (abbreviated as apoE23) with the sequence of LRKLRKRLVRLASHLRKLRKRLL, which exhibited high antibacterial effects. The minimal inhibitory concentration of apoE23 against multidrug‐resistant Acinetobacter baumannii was 6 µg/ml. The antimicrobial activity of apoE23 depended on its amphipathic α ‐helical conformation. Moreover, apoE23 downregulated the expression of tumour necrosis factor‐ α , interleukin‐6 and interleukin‐10 in lipopolysaccharide‐induced THP‐1 cells. ApoE23 exhibits potential in future clinical applications. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.