Premium
A novel glucagon‐like peptide 1 peptide identified from Ophisaurus harti
Author(s) -
Zhu Jingjing,
Huang Xian,
Gao Hong,
Bao Qiuying,
Zhao Yun,
Hu JinFeng,
Xia Gang
Publication year - 2013
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2538
Subject(s) - peptide , agonist , chemistry , receptor , high throughput screening , biochemistry
Glucagon‐like peptide 1 receptor (GLP1R) is a promising target for the treatment of type 2 diabetes. Because of the short half‐life of endogenous GLP1 peptide, other GLP1R agonists are considered to be appealing therapeutic candidates. A high‐throughput assay has been established to screen for GLP1R agonists in a 60 000‐well natural product compound library fractionated from 670 different herbs/materials widely used in traditional Chinese medicines (TCMs). The screening is based on primary screen of GLP1R + reporter gene assay with the counter screen in GLP1R − cell line. An active fraction, A089‐147, was identified from the screening. Fraction A089‐147 was isolated from dried Ophisaurus harti , and the fact that its GLP1R agonist activity was sensitive to trypsin treatment indicates its peptidic nature. The active ingredient of A089‐147 was later identified as O. harti GLP1 through transcriptome analysis. Chemically synthesized O. harti GLP1 showed GLP1R agonist activity and sensitivity to dipeptidase IV digestion. This study illustrated a comprehensive screening strategy to identify novel GLP1R agonists from TCMs libraries and at the same time underlined the difficulty of identifying a non‐peptidic GLP1R agonist. The novel O. harti GLP1 peptide yielded from this study confirmed broader application of TCMs libraries in active peptide identification. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.