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Multiple antigen peptide mimetics containing gp41 membrane‐proximal external region elicit broad neutralizing antibodies against human immunodeficiency virus type 1 in guinea pigs
Author(s) -
Zhang Lishuang,
Miao Liang,
Gong Xin,
Zhang Huayan,
Yang Lan,
Shi Yuhua,
Kong Wei,
Jiang Chunlai,
Shan Yaming
Publication year - 2013
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2526
Subject(s) - epitope , adjuvant , monoclonal antibody , antibody , peptide , virology , gp41 , neutralizing antibody , antigen , human immunodeficiency virus (hiv) , chemistry , biology , virus , immunology , biochemistry
Eliciting a broadly neutralizing antibody response against the HIV‐1 membrane‐proximal external region (MPER) mimicking the activity of 4E10 and 2F5 monoclonal antibodies remains a major challenge. In this study, two novel tetra‐branched peptide immunogens, 4E10‐ and 2F5‐MAP4, were designed and synthesized using a MAP system. Guinea pigs were immunized with either of these two synthetic immunogens emulsified in an oil‐phase adjuvant at 3‐week intervals. After four immunizations, epitope‐specific antibody responses were induced successfully, and moderate neutralizing activities against tier 1 (clades B, BC, AE) and tier 2 (clade C) HIV‐1 pseudoviruses were detectable in unfractionated sera and purified IgGs. The synthetic gp41 membrane‐proximal external region peptide mimetics, 4E10‐ and 2F5‐MAP4, assisted by an appropriate adjuvant, are promising prophylactic vaccine candidates potentially capable of eliciting broadly neutralizing antibody responses against HIV‐1. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.